Tuesday, February 2, 2010

SAFETY AND EFFICACY OF ANTI-PHOSPHOLIPASE A2 ANTIBODIES PRODUCT (Big Fish) AS A FEED SUPPLEMENT ON GROWTH OF JUVENILE VIETNAMESE CATFISH

Mingder Yang*, Kyle Montgomery and John S. Clark

AovaTechnologies, Inc.
3513 Anderson St. Ste 100
Madison, WI 53704
mingder@aovatech.com The technology involves the use of antibody product to the target phospholipase A2 (PLA2) enzyme. The antibody to the enzyme PLA2 (aPLA2) is thought to decrease the inflammatory response when lumen bacteria interact with intestinal cells. PLA2 is a key host enzyme that involves in cascade of producing prostaglandins and leukotrienes, which are potent inflammatory mediators. By blocking the PLA2 enzyme, the antibody in feed can minimize the overwhelming inflammation in the intestine when bacteria interact with gut lining cells. The end result is more efficient utilization of available energy and nutrients for growth and performance instead of resource allocation to an inflammation response.

The protocol is designed to assess the impact of Big Fish on a number of biological parameters in cultured Vietnamese Catfish. Catfish in Vietnam is considered an important food fish, with over 1 million MT produced annually. Of this total, 50% is consumed locally, but the balance 50% is exported as tra or basa fillets, mainly to the United States. Export demand for larger fish is rising rapidly, and industry growth estimates of 20% increase per annum are not uncommon.

In the current trial, there were three treatment groups: control, and 0.25% or 0.50% Big Fish supplementation. Within each treatment group there were 5 replicates, each of which contained 100 fish of initial weight 20grams. The trial ran for a period of 90 days and took place in the research facility of a commercial catfish hatchery and nursery complex located in the province of Chonburi (just outside Chachoengsao City), about 1 hours drive east from Bangkok, Thailand.

Big Fish groups had a better survivability starting in week 2. By the end of week 13, Big Fish significantly improved survival of catfish by 29% and 46% over control with 0.25% and 0.5% Big Fish supplement respectively. Weekly weight gain showed difference as early as week 2. By week 13, the weight gain improvement for Big Fish groups were 136 grams for 0.25% and 144 grams for 0.5% supplement group compared to 125 grams for control group. When combined improved survivability and increased weight gain, total biomass was improved 39% and 65% by 0.25% and 0.5% Big Fish supplement over control group in 13-week trial. Feed conversion ratio (FCR) did not differ until week 3 and by the end of trial, feed efficiency was improved 20% and 26.3% by 0.25% and 0.5% Big Fish supplementation, respectively.

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